EnzymeQA2

EnzymeQA2 contains revision questions and answers on enzyme catalysis.

EnzymeQA2 is an App containing about 160 revision questions and answers on enzymology. It is restricted to considering single substrate catalysis. It is hoped that it will help University undergraduate and postgraduate students revise enzymology at home or on the go. It is not an exhaustive treatment of enzymology. However, it is hoped that it will enable you to test your knowledge of key facts in enzymology.



For those of you planning to determine kcat, kcat/Km and Km, EnzymeQA2 should help you check that you have the appropriate experimental conditions for determining catalytic constants. Likewise it should help planning to determine Ki values for competitive inhibition.



EnzymeQA2 contains 5 windows which give questions and answers on the following topics:-

Enzyme kinetics

Enzyme inhibition

Effect of pH on enzyme kinetics

Enzyme assays

Transition state stabilisation in catalysis

Topics in EnzymeQA2 include:-


ENZYME KINETICS WINDOW

Michaelis Menten equation.

Initial rate measurements.

Setting the appropriate concentrations of enzyme and substrate for determining catalytic parameters.

Defining Vm, kcat, Km, kcat/Km, Ki and IC50 values.

Units of catalytic constants.

Order in enzyme and substrate at various substrate concentrations.

Methods for determining catalytic constants.

Experimental conditions for measuring catalytic constants.

ENZYME INHIBITION WINDOW

Competitive inhibition.

Determining Ki values for competitive inhibition.

Relationship between Ki and IC50 values.

Brief mention of noncompetitive, mixed and uncompetitive inhibition.

EFFECT OF PH ON ENZYME KINETICS WINDOW

pH and pH values

Analysis of pH data

log plots

pH dependence of catalytic constants.

pKa perturbation in carbonic anhydrase.

ENZYME ASSAYS WINDOW

Elisa

Enzyme cycling

Advantages and disadvantages of spectrophotometric and fluorescence assays

FRET assays

Using plate readers for enzyme assays

Stopped flow spectrophotometry

TRANSITION STATE STABILISATION IN CATALYSIS WINDOW

Transition state analogues

Catalytic antibodies

Ribozymes